The primary quantification targets small autosomal and y consist of relatively short amplicons 75 to 80 bases to improve the detection of degraded dna. Life technologies quantifiler duo dna quantification kit, which is the quantification kit. Portable patient monitor, including circuit descriptions, test and calibration procedures, and spare parts listings. Developmental validation of the quantifiler duo dna quantification kit for simultaneous quantification of total human and human male dna and detection of pcr inhibitors in biological samples. Reagent kit selection guide biochain institute inc. Foster city, ca, using the procedures described in the user manual accompanying the kit. See the complete profile on linkedin and discover michaels.
View michael kochs profile on linkedin, the worlds largest professional community. Direct qpcr quantification using the quantifiler trio dna. Applied biosystems in the quantifiler duo users manual which states the following. Quantifiler duo dna quantification kit user guide pub.
It is a simple one to one video calling application. The quantifiler duo dna quantification kit has the added advantage of being. Quantifiler duo dna quantification kit using the ab 7500. Orientation the intimidator spot duo may be mounted in any position. Quantifiler human dna standard both quantifiler kits utilize the same reagents and allow the user to select the appropriate quantifiler primer mix, which contains a preformulated mixture of primers and probes that amplify either the human telomerase reverse transcriptase gene quantifiler human kit or the sry gene quantifiler y kit. Trusted endpoints identify managed devices and block unknown device access duo beyond features.
The effectiveness of a direct quantification assay is essential to the adoption of the combined direct quantificationdirect str workflow. Validation of quantifiler trio dna quantification kit in forensic samples. A guiding tool for short tandem repeat genotyping of forensic samples. Quantifiler duo dna quantification kit for simultaneous. Information regarding preparation of standards should be recorded in quantifiler duo dna quantification standards log. My book duo user manual about your wd storage device 1 about your wd storage device my book duo premium raid storage provides the quickest way to transfer, save and protect your photos, videos, music and other massive files to free up valuable space on your computers. Validation of prepfiler forensic dna extraction kit.
The 2x master mixes of both the quantifiler duo and s. The quantifiler duo dna quantification kit enables forensic laboratories to simultaneously obtain a quantitative and qualitative assessment of total human and human male dna in a single, highly sensitive realtime pcr reaction. Standards should be stored from 25c and expire after one week. Lastpass supports multifactor authentication with duo security. Duo beyond includes all duo access and mfa features duo beyond overview a complete zerotrust security platform duo beyond features. In this paper, the feasibility of using the quantifiler trio dna quantification kit for the direct quantification of forensic casework samples was investigated. Validation of automated pcrsetup of the quantifiler trio. A sample extraction method for faster, more sensitive pcr. Quantifiler duo dna quantification kits users manual, pn 4391294 rev. The reproducibility of quantification results for total human dna sa and male dna y was tested, using the following dilution series of one male sample. Buffalo linkstation duo network storage quick start guide. Your email address will never sold or shared with anyone else without your express permission.
Assessing and troubleshooting false positive results. The manufacturers user manual does not recommend lysozyme or proteinase k treatment, so none was performed. Duo service manual 007010060401 iii foreword introduction. Pool ed human male genomic dna is used to generate standard curves for both. Pdf forensic analysts routinely encounter samples containing mixtures of dna from male and female contributors and pcr inhibitors due to exposure to. Google in its first innings with duo, has primarily focused on three major aspects. While the quantifiler kit users manuals provide detailed information regarding. View julie casals profile on linkedin, the worlds largest professional community.
The assay provides data for downstream selection of genotyping with autosomal, y or mtdna 42. Quantifiler duo dna quantification kit thermo fisher scientific. Quantifiler duo dna quantification kit user guide chapter 1 overview realtime data analysis 1 the 7500 system uses the data obtained from the pure. One, quantifiler duo, utilizes a taqman assay with single copy probes for both. The quantifiler duo dna quantification kit enables forensic laboratories to simultaneously obtain a quantitative and qualitative assessment of total human and. In the present study we tested the performance of prepfiler kit against other commonly used commercially available kits on a variety of real forensic casework samples. This article appeared in a journal published by elsevier. Quantification kits, these kits use taqman quantitative. Part number 4391294 rev 042008 part number 4391294 rev 042008 barbisin, maura, et al. All samples were than quantified in realtime pcr by a 7300 realtime pcr system, using the quantifiler duo dna quantification kit that simultaneously obtain a quantitative and qualitative assessment of total human and human male dna and permits, to verify the presence of pcr inhibitors. The quantifiler kits, and in particular the quantifiler duo kit, provides a comprehensive assessment of the content, quantity, and.
West virginia state police dna analysis procedures manual, that protocol was. Str loci including the loci in the european minimal haplotype dys19. Quantifiler human dna quantification kit quantifiler y human. Submit your questions at everyone wants to know how much dna is in their extract, but then they ask. A small set of mock casework nonbone samples were also generated and extracted inhouse. The quantifiler trio dna quantification kit is a robust kit that enables forensic laboratories to simultaneously obtain a quantitative and qualitative assessment of total human and human male dna in a single, highly sensitive realtime pcr reaction. Quantifiler hp and trio dna quantification kits user guide. Developmental validation of globalfiler pcr amplification. Additional items optional may be included into the package, depending on player configuration, region, and distributor.
Quantifiler duo and plexor hy for detecting and quantitating biological evidence. Part of the work was presented at the 59th annual meeting of the american academy of forensic sciences, in san antonio, tx, february 1924, 2007, and at the 18th international symposium on human identification, hollywood, ca, october 2007. It belongs to human identification solutions category. Foster city, ca using the procedure described in the users manual for each. Particularly, we tested the reproducibility of the kit and its performance on lowtemplate samples, focusing. Sep 17, 2009 the prepfiler is a new kit recently introduced by applied biosystems for dna extraction from a wide range of forensic samples. The internal validation of life technologies quantifiler trio and. Validation of prepfiler forensic dna extraction kit applied. This manual is intended as a guide for technically qualified personnel during repair. Have the duo admin go to duo admin panel users tab click on your account and remove your phone number under phones.
Duo network gateway give users ssh and web access to internal apps and hosts without a vpn duo beyond features. The automate express system generated eluates that were approximately 50. The developed assay enables the assessment of the biological samples for downstream str profiling. There are no user serviceable components within this equipment. Join more than 55,000 people who get scomm and ubiduo news, promotions and success stories in their inbox each month we hate spam too. Page 10 of 28 intimidator spot duo user manual mounting before mounting the product, read and follow the safety recommendations indicated in the safety notes.
Number of tests 48 extractions kit components kit contents z2201048 reagent cartridge 48 pcs reaction chamber 48 pcs tip holder 48 pcs. The prepfiler is a new kit recently introduced by applied biosystems for dna extraction from a wide range of forensic samples. It is a secure, twofactor authentication application offered for all leading smartphone platforms, including android, iphone, blackberry, and windows phone. Dfs fsl fbu validation summary report for quantifiler duo using the 7500 page 2 of 6 concentration difference 2. Strs in forensic laboratories, especially in cases where typing autosomal strs has met with limited success. Development and forensic validation of human genomic dna. The eos net duo come with male 4mm bullet connectors aka banana plugs attached to the power input cables. Since automation of pcrsetups reduces the manual workload and the risk for human errors, we have chosen to validate the pcrsetup of the quantifiler trio kit on a small benchtop liquid handler, the biomek 4000 beckman coulter. The quantifiler duo dna quantification kit enables simultaneous quantification of human dna and human male dna as well as detection of inhibitors of pcr in a single realtime pcr well. The quantifiler duo dna quantification kit enables simultaneous. Disable duo authentication for your lastpass account.
These cables are appropriate for attaching directly to most highquality acdc power supply units, such as the bk precision usa model 169215v, 40a, 600w. Julie casals criminalist indian river crime laboratory. Enrolling users adding users to duo introduction to duo. More recent qpcr kits that have been widely adopted in forensic dna laboratories include quantifiler duo and plexor hy. As with our quantifiler duo, human, and y human male dna. Vehicle accident camera with 120 wide angle lens and additional rear view internal camera. Michael koch owner michael koch consulting linkedin. Quantifiler duo dna quantification kit user guide quantifiler duo dna quantification kit user guide quantifiler duo dna quantification kit user guide. Quantifiler hp and trio dna quantification kits user guide pub. Silva rha, musse jo, melani rfh, oliveira rn, human bite mark identification and dna technology in forensic dentistry, braz j oral sci.
In the past 5 years, there has been a substantial increase in the use of y. Manuals originating from over 40 forensic dna crime laboratories and private dna testing facilities spanning across the united states. Quantifiler duo dna quantification kit users manual. Quantifiler trio kit and forensic samples management. This service manual gives a detailed description of the. All of the samples were quantified according to the manufacturers instructions. Quantifiler duo dna quantification kit, for simultaneous quantification of human nuclear and human male dna as well as detection of the presence of pcr inhibitors in a biological sample. Developmental validation of the quantifilera duo dna. Validation of quantifiler trio dna quantification kit in forensic. The quantifiler duo dna quantification kit enables simultaneous quantification of human dna and human male dna as well as detection of inhibitors of pcr in. Application note quantifiler duo dna quantification kit utilizing the quantifiler. If you have switched phones or duo tokens, follow the steps below to reset up your lastpass account with duo. The quantifiler kits were tested for performance criteria such as species. See quantifiler duo dna quantification kit users manual for information regarding the procedure.
Log into your lastpass account and reenable duo authentication. The eos netduo come with male 4mm bullet connectors aka banana plugs attached to the power input cables. Quantifiler duo dna quantification kit user manual. Quantifiler duo dna quantification kit, user manual. Duo is a onetoone video calling app for everyone designed to be simple, reliable and fun so you never miss a moment. The dna was quantified using the quantifiler duo dna quantification kit on an applied biosystems 7500 realtime pcr system thermo fisher scientific. Quantifiler duo dna quantification kit product information sheet. Some of the data is also presented in the quantifiler duo dna quantification kit user manual.
Audience this manual is intended for scientists who use the quantifiler duo dna quantification kit for the quantification of human dna extracted from a variety of sample types. Users manual respirator fit tester and software for windows 2000 and xp and vista for help visit our web site or call. Quantifilerduo dna quantification kit omics international. Developmental validation of the quantifiler duo dna. To make sense of the raw data, the software determines.109 868 1007 613 178 132 1361 1383 799 844 384 875 518 397 1208 710 645 879 1467 1249 520 68 872 1508 1137 824 117 237 605 1306 997 1212 288